@article{Красова_Караченцев_Горшунська_Колеснікова_Лещенко_Гладких_Воропай_Романова_Полторак_2021, title={THE ROLE OF LEPTIN AND LEPTIN RECEPTOR GENE POLYMORPHISM IN THE OMEGA-3 POLYUNSATURATED FATTY ACIDS METABOLIC EFFECT REALIZATION IN TYPE 2 DIABETIC PATIENTS OF THE EAST UKRAINIAN POPULATION}, volume={78}, url={https://jpep.endocrinology.org.ua/index.php/1/article/view/844}, DOI={10.21856/j-PEP.2021.4.04}, abstractNote={<p>It is known that single nucleotide polymorphisms (SNPs) in the adipokine genes can affect both the development of pathological conditions associated with obesity and the effectiveness of pharmacotherapy. <strong>Aim.</strong> To evaluate the impact of polymorphic variants for leptin gene <em>LEP 2548G&gt;A</em> (rs7799039) and leptin receptor gene <em>LEPR 223G&gt;A</em> (rs1137101) on the omega-3 polyunsaturated fatty acids therapeutic efficacy realization in patients with type 2 diabetes mellitus of the East Ukrainian population. A total of 61 patients with type 2 diabetes mellitus (DM) (m/f 34/27; age 53.4 ± 1.4 yrs, diabetes duration 5.3 ± 0.7 yrs; fasting blood glucose 9.1 ± 0.4 mmol/l; HbA1c 7.7 ± 0.2%) overweight or obese (body mass index 33.3 ± 0.9 kg/m<sup>2</sup>) were received in addition to standard oral hypoglycemic therapy (sulfonylurea and/or metformin) daily for 3 months the omega-3 polyunsaturated fatty acids (PUFA) (460 mg eicosapentaenoic acid + 380 mg docosahexaenoic acid). The control group consisted of 21 subjects. Clinical and biochemical parameters were assessed before and after the treatment. Genotyping by SNP <em>LEP 2548G&gt;A</em> and <em>LEPR 223G&gt;A</em> was performed using the polymerase chain reaction method with appropriate primers and endonucleases (<em>HhaI</em> and <em>MspI</em>). <strong>Results.</strong> It was determined that type 2 DM patients carriers of the dominant G-allele (SNP <em>LEP 2548G&gt;A</em>) show greater effectiveness of omega-3 PUFA in reducing of total insulin resistance and adipose tissue insulin resistance. The association of recessive genotype (<em>AA</em>) by SNP <em>LEPR 223G&gt;A</em> with greater efficiency of omega-3 PUFA in reducing adipose tissue insulin resistance was revealed. The studied polymorphisms of adipokine genes have an impact on the implementation of therapeutic hormonal and metabolic efficacy of omega-3 PUFA under 3-month administration in patients with type 2 DM and overweight/obesity of the East Ukrainian population. Data on polymorphic variants of the LEP and/or LEPR gene can be used within pharmacogenetics as a basis for personalized prophylaxis and therapy with a predicted effect of the pharmacological intervention.</p>}, number={4}, journal={Problems of Endocrine Pathology}, author={Красова, Наталія and Караченцев, Юрій and Горшунська, Мар’яна and Колеснікова, Алла and Лещенко, Жанна and Гладких, Олександр and Воропай, Тетяна and Романова, Ірина and Полторак, Вікторія}, year={2021}, month={Dec.}, pages={27-33} }