EXPERIMENTAL SUBSTANTIATION OF JAW BONE TISSUE DYSMETABOLISM PREVENTION IN RATS WITH HYPOTHYROIDISM
Keywords:thyroid insufficiency, alveolar process, bone remodeling, prevention
Thyroid insufficiency leads to severe consequences in all organs and systems, including bone tissue. Disorders of bone remodeling in this pathology have not been studied enough. Meanwhile, timely prevention is important to prevent the progression of jaw bone loss and periodontitis in iodine-deficient regions.
Therefore, the aim of the study was to investigate the effectiveness of prevention of remodeling disorders in the bone tissue of the alveolar process of the jaws of rats with hypothyroidism.
Materials and methods. The studies were performed on 36 female and male Wistar rats, which were divided into three groups (6 females and 6 males): 1) intact, 2) model of hypothyroidism and 3) prevention of hypothyroidism with a complex of vitamins and minerals. Hypothyroidism was simulated by oral administration of 1% potassium perchlorate solution. The duration of the experiment was 4 months. After euthanasia in the jaws of animals evaluated the degree of atrophy of the alveolar process, indicators of destruction (elastase and acid phosphatase activity) and osteogenesis (alkaline phosphatase activity), the state of the antioxidant system (catalase activity, superoxide dismutase and glutathione reductase) and lipid peroxidation (malonic dialdehyde content) in the bone tissue of the jaws. The composition of the preventive agent is represented by a complex of macro- and microelements (selenium, manganese, copper, magnesium and calcium), vitamins C and D, quercetin.Results. Studies have shown that hypothyroidism leads to an increase in alveolar atrophy, increased resorption of rat bone tissue (increased acid phosphatase and elastase activity) and inhibition of osteosynthesis (decreased alkaline phosphatase activity). Disturbances in the state of the antioxidant-prooxidant system of bone tissue of rat jaws were also revealed: the activity of superoxide dismutase and glutathione reductase of bone tissue was compensatory increased, and catalase, on the contrary, was depleted. As a result, a significant amount of toxic malonic dialdehyde accumulated in the bone tissue of the jaws during hypothyroidism.Introduction of prophylactic complex to rats with hypothyroidism led to inhibition of alveolar atrophy, decreased bone acid phosphatase and elastase activity, increased alkaline phosphatase activity and normalization of antioxidant enzyme activity and malonic dialdehyde content. Conclusion. The obtained results are impressive about the pronounced osteoprotective and antioxidant properties of the complex of vitamins and minerals in conditions of thyroid insufficiency.
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