HORMONE PROFILE OF OVULATION
DOI:
https://doi.org/10.21856/j-PEP.2025.2.03Keywords:
ovulation, hormonal profile, cryotransfer, ovulation markers, sex hormonesAbstract
In order to increase the efficiency of in vitro fertilization (IVF), it is necessary to determine the exact time of follicle rupture, which is important for improving cryotransfer protocols and the woman's hormonal profile.
The purpose of the study is to study hormonal changes in a woman's body during ovulation, in order to establish the exact time of follicle rupture to increase the effectiveness of cryotransfer.
Materials and methods. 50 women were under observation, in which euploid embryos were stored in the cryobank. The average age of the patients was 34.7±0.6 years. Every day, starting from the 10th day of the cycle, the dynamics of follicle growth and the time of its rupture were observed. The blood concentration of progesterone, estradiol, and luteinizing hormone (LH) was studied by enzyme-linked immunosorbent assay using reagent kits manufactured by DRG Instruments GmbH (Germany) on all examination days. The nonparametric Wilcoxon paired test (Z; p) for related samples was chosen as the comparison criterion.
Results. The average level of each hormone significantly (p<0.05) differs by day. The levels of three sex hormones were used as predictors: progesterone, LH, and estradiol. On their basis, a discriminant model with high statistical significance of discriminant functions was obtained. Theoretically, any of these three hormones can be used as a marker for diagnosing ovulation, but estradiol and progesterone were chosen to determine the hormonal marker of ovulation (HMO). A ROC curve was obtained, which indicates its excellent discriminant ability in determining the presence of ovulation. The threshold values of the indicators are given, which correspond to a certain sensitivity and specificity of the criterion for determining the day of the cycle.
Conclusions. Based on a comprehensive approach with hormonal assessment and ultrasound examination, a hormonal marker of ovulation has been developed and its threshold value has been determined. The data obtained will help determine the day of embryo transfer to the endometrium, the most favorable for implantation, which, in turn, will increase the chance of pregnancy.
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