MORPHOLOGICAL FEATURES OF THE INFLUENCE OF CORD BLOOD BIOPREPARATION ON THE THYROID GLAND OF RATS WITH EXPERIMENTAL AUTOIMMUNE THYROIDITITIS

Authors

DOI:

https://doi.org/10.21856/j-PEP.2022.2.11

Keywords:

thyroid gland, autoimmune thyroiditis (AT), thyroiditis (HT), Cord Blood Serum (CBS), biopreparation

Abstract

Abstract

Relevance. One of the modern medical trends of the 21st century is regenerative medicine – a clinical discipline that aims to use cell therapy to repair damaged organs and tissues. Experimental and clinical studies have proven the great restorative potential of fetoplacental complex (FPC) biological products which is due to a wide range of biologically active substances which determines the prospects and relevance of their study in many laboratories around the world in recent decades.

Purpose of research. Determination of histomorphological changes in the thyroid gland which occur during the simulation of experimental autoimmune thyroiditis and after correction of this pathology by a non-cellular preparation of cord blood serum in the early stages of its aftereffect study.

Materials and methods. Experimental studies were performed on male Wistar rats with a simulated autoimmune thyroiditis (AT). Administration of the drug "Cryocell-Cryocord" was carried out intramuscularly at a rate of 10 injections every other day at the rate of 0.1 ml of diluted solution per 100 g of body weight. The comparison drug "Levothyroxine" (Berlin-Chemie, Germany) was administered to rat at a dose of 10 μg/kg of the body weight for 10 days. Histological specimens were analyzed using a Primo Star hardware and software complex, a Canon power shwet ATIO camera, a personal computer, and the Morpholog program.

Results. Histomorphological analysis of the thyroid gland of rats, after 1 month. after the creation of the model, AIT confirms the stable development of its autoimmune lesions: significant foci of lymphocytic infiltration and connective tissue strands were observed. "Levothyroxine" and "Cryocell-Cryocord" had a positive effect on the histostructure of the rat thyroid gland. The more pronounced effect was observed after the introduction of the biological product "Cryocell-Cryocord". Under its influence, more significant foci were identified in the thyroid gland with signs of normalization of structural elements and restoration of its functional activity.

Conclusions. On the basis of morphological and morphometric studies, a conclusion was made about the high efficiency of the use of the cell-free biological product "Cryocell-Cryocord" for the correction of autoimmune damage to the thyroid gland.

Author Biography

Kurylko Yu. S., SI "V. Danilevsky Institute for Endocrine Pathology Problems of the NAMS of Ukraine", Kharkiv, Ukraine

Kurylko  Yuliia  Serhiivna, born in 1991, graduated in 2013 from the Kharkiv National Pedagogical University. GS Skovoroda, Faculty of Natural Sciences, specialty "Biology", qualification-master. From 2012 to the present time have been working in the laboratory of pharmacology, the department of experimental pharmacology and toxicology of State Institution "V. Danilevsky Institute for Endocrine Pathology Problems of the NAMS of Ukraine"as a laboratory assistant of the 1st category. September 2, 2019 entered the postgraduate course of the State Institution "V. Danilevsky Institute for Endocrine Pathology Problems of the NAMS of Ukraine"

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Published

2022-06-09

How to Cite

Kurylko, Y. (2022). MORPHOLOGICAL FEATURES OF THE INFLUENCE OF CORD BLOOD BIOPREPARATION ON THE THYROID GLAND OF RATS WITH EXPERIMENTAL AUTOIMMUNE THYROIDITITIS. Problems of Endocrine Pathology, 79(2), 72-79. https://doi.org/10.21856/j-PEP.2022.2.11