THYROID FUNCTION OF RATS WITH EXPERIMENTAL AIT AT AN EARLY PERIOD AFTER THE INJECTION OF BIOPREPARATIONS OF CORD BLOOD

Authors

DOI:

https://doi.org/10.21856/j-PEP.2021.4.14

Keywords:

autoimmune thyroiditis, thyroid gland, Cryocell-Cryocord

Abstract

The development of new methods for the treatment of autoimmune thyroid disease is an actual problem of endocrinology. The aim of research was studying of the cell-free effect biological product of cord blood with immunomodulatory properties "Cryocell-Cryocord " on rats’ thyroid function with AIT simulated at an early period of the study. Simulation of autoimmune thyroiditis was carried out by immunization with a suboperatively isolated human thyroid antigen in combination completing Freund's adjuvant on 30 adult male rats weighing 130 - 150 g. Correction was carried out by parenteral ingection of the "Cryocell-Cryocord". Hormonal research (determination of the content of thyroid hormones and antibodies to thyroglobulin (TG-AT) were effected by using standard test kits for enzyme-linked immunosorbent assay at early stages after exposure (7 days, 1 month). Statistical maintained of the results was carried out using the methods of variation statistics. It was used normal distribution to unpaired Student's test to compare indicators. The difference was considered significant at p<0.05. It has been shown the biological preparation of cord blood "Cryocell - Cryocord" has a positive effect with induced autoimmune thyroiditis. It has been noted the normalizing result of the effected correction on the thyroid function of animals in the early period of the studying. It has been proved after the introduction of the biological preparation "Cryocell - Cryocord" in animals with experimental autoimmune thyroiditis observed the inhibition of the manifestations of autoimmune aggression and the normalization of the functional activity of the thyroid gland. These investigations can be the foundation for the development of a new approach to the treatment of patients.

Author Biography

Kurylko Yu. S., SI "V. Danilevsky Institute for Endocrine Pathology Problems of the NAMS of Ukraine", Kharkiv, Ukraine

Kurylko  Yuliia  Serhiivna, born in 1991, graduated in 2013 from the Kharkiv National Pedagogical University. GS Skovoroda, Faculty of Natural Sciences, specialty "Biology", qualification-master. From 2012 to the present time have been working in the laboratory of pharmacology, the department of experimental pharmacology and toxicology of State Institution "V. Danilevsky Institute for Endocrine Pathology Problems of the NAMS of Ukraine"as a laboratory assistant of the 1st category. September 2, 2019 entered the postgraduate course of the State Institution "V. Danilevsky Institute for Endocrine Pathology Problems of the NAMS of Ukraine"

References

Antonelli A, Ferrari SM, Corrado A, et al. Autoimmun Rev 2015;14(2): 174-180. https://doi.org/10.1016/j.autrev.2014.10.016.

Tkachenko VI. Mizhnar Endokrynol Zhurn 2018;14(3): 279-284. available at: http://nbuv.gov.ua/UJRN/Mezh_2018_14_3_14.

Dedov II, Mel'nichenko GA. Jendokrinologija, Moskva, 2016: 1112.

Gardner D, Gardner D, Shobek D. Bazisnaja i klinicheskaja jendokrinologija, Moskva, 2016;1: 464 р.

Feldkamp J. Dtsch Med Wochenschr 2009;134(49): 2504-2509. https://doi.org/10.1055/s-0029-1243053.

Farkas G, Dégi R, Vörös P. Transplant Proc 1995;27(6): 3145.

Da Silva Meirelles L, Chagastelles PC, Nardi NB. J Cell Sci 2006; 119(11): 2204-2213. https://doi.org/10.1242/jcs.02932.

Caplan AI, Dennis JE. J Cell Biochem 2006;98(5): 1076-1084. https://doi.org/10.1002/jcb.20886.

Gol'cev AN. Visn NAN Ukrai'ny 2015;5: 35-37.

Kovalov GA, Ischenko IO, Tynynyka LN, et al. Probl Cryobiol Cryomed 2017;27(1): 71-80.

Scherbina MA, Potapova LV, Scherbina IN, Musatova IB. Probl Cryobiol Cryomed 2008;18(3): 364-365. available at: http://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/412.

Vvedenskiy BP, Kovalev GA, Dedukh NV, Sandomirskiy BP. Probl Cryobiol Cryomed 2016;26(4): 361-374. available at: http://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/1245.

Poljachenko JuV, Nikonenko OS, Saljutin GV, et al. Klitynna Transplantacija 2013;1(1): 40-43 .

Grishhenko VІ. Problemy Kriobiologii 2008;18(3): 269-274.

Chernjavskaja EA. Pryrodnychi nauky: istorija, suchasnist', majbutnje, dosvid JeS: materialy mizhnar. nauk.-prakt. konf., Vloclavek, 27-28 veres 2019: 147-150.

Reznikov OG. Endokrynologija 2003;8(1): 142-145.

Downloads

Published

2021-12-03

How to Cite

Kurylko, Y., Malova, N., & Sirotenko, L. (2021). THYROID FUNCTION OF RATS WITH EXPERIMENTAL AIT AT AN EARLY PERIOD AFTER THE INJECTION OF BIOPREPARATIONS OF CORD BLOOD. Problems of Endocrine Pathology, 78(4), 104-109. https://doi.org/10.21856/j-PEP.2021.4.14

Most read articles by the same author(s)