COMPARISON OF THERAPEUTIC EFFECTS OF DIFFERENT METHODS OF ADMINISTRATION OF MEZENCHIMAL STEM CELLS TO MICE WITH PREMATURE OVARIAN INSUFFICIENCY

Authors

  • Kozub M. I. Kharkiv Medical Academy of Post-Graduate Education, Kharkiv, Ukraine
  • Skybina K. P. Kharkiv Medical Academy of Post-Graduate Education, Kharkiv, Ukraine
  • Musatova I. B. Institute for Problems of Cryobiology and Cryomedicine NAS of Ukraine, Kharkiv, Ukraine
  • Prokopiuk O. V. Kharkiv Medical Academy of Post-Graduate Education, Kharkiv, Ukraine
  • Gramatiuk S. M. Kharkiv Medical Academy of Post-Graduate Education, Kharkiv, Ukraine
  • Tynynyka L. M. Private institution of higher education «Kharkiv international medical university», Kharkiv, Ukraine
  • Kozub M. M. Kharkiv Medical Academy of Post-Graduate Education, Kharkiv, Ukraine
  • Taran A. V. National University of Pharmacy, Kharkiv, Ukraine
  • Nikolchenko A. Yu. Private institution of higher education «Kharkiv international medical university», Kharkiv, Ukraine
  • Prokopiuk O. S. Institute for Problems of Cryobiology and Cryomedicine NAS of Ukraine, Kharkiv, Ukraine

DOI:

https://doi.org/10.21856/j-PEP.2021.2.05

Keywords:

premature ovarian insufficiency, mesenchymal stem cells, adipose tissue, chemotherapy.

Abstract

Premature ovarian insufficiency (POI) is condition of female hypogonadism. One of the reasons for the development of POI is anticancer chemotherapy. Currently, hormone replacement therapy is considered the most effective method of treatment POI, which, however, had a number of side effects, requires constant administration of pharmaceuticals, did not restore fertility and correct pathological changes in target organs. Therefore, the search for alternative methods of treatment based on the use of stem cells is promising. The aim of work was to compare the therapeutic effects of different methods of administration of mesenchymal stem cells isolated from adipose tissue (AT MSCs) in restoring sexual function, as well as the behavior of mice with chemotherapy-induced premature ovarian failure.

Materials and methods. The investigation was carried out on BALB/c mice with a POI model induced by Cyclophosphamide and Busulfan. A histological examination of preparations of the ovaries and uterus was carried out. We studied the changes in the weight of mice, ovarian function, sexual activity, as well as the behavior of animals in anxiolytic and zoosocial tests after treatment with AT MSCs with intraperitoneal and intraovarian routes of administration.

Results and discussion. It was shown that the effectiveness of the use of AT MSCs depends on the method of their administration at the treatment of POI induced by chemotherapy. More rapid and complete restoration of the morphological structure, sexual function, and behavioral responses were observed in mice with POI treated by intraovarial administration of AT MSCs.

 

References

Na J, Kim GJ. J Ovarian Res 2020;13(1): 74. https://doi.org/1186/s13048-020-00671-2.

Ding C, Li H, Wang Y, et al. Stem Cell Res Ther 2017;8: 173. https://doi.org/10.1186/s13287-017-0613-3.

Fenton AJ. J Midlife Health 2015;6(4): 147-153. https://doi.org/10.4103/0976-7800.172292.

Kozub MM, Prokopiuk VY, Skibina KP, et al. Exp Oncol 2017;39(3): 181-185.

Dah-Ching Ding, Woei-Cherng Shyu, Shinn-Zong Lin. Cell Transplant 2011;20(1): 5-14. https://doi.org/10.3727/096368910X.

Lelek J, Zuba-Surma EK. Int J Mol Sci 2020; 21(3): 799. https://doi.org/10.3390/ijms21030799.

Musatova IB, Volina VV, Chub OV, et al. Neurophysiology 2017; 49(5): 363-371.

Xiao GY, Liu IH, Cheng CC, et al. PLoS One 2014; 9(9). https://doi.org/10.1371/journal.pone.0106538.

Sun M, Wang S, Li Y, et al. Stem Cell Res Ther 2013;4(4): 80. https://doi.org/10.1186/scrt231.

Himanshu, Dharmila, Sarkar D, Nutan. Clin Psychopharmacol Neurosci 2020; 18(3): 341-351. https://doi.org/10.9758/cpn.2020.18.3.341.

Heifets BD, Salgado JS, Taylor MD, et al. Sci Transl Med 2019;11(522): 6435. https://doi.org/10.1126/scitranslmed.aaw6435.

Vichaya EG, Chiu GS, Krukowski K, et al. Front Neurosci 2015;9: 131. https://doi.org/10.3389/fnins.2015.00131.

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Published

2021-06-10

How to Cite

Kozub, M. I., Skybina, K. P., Musatova, I. B., Prokopiuk, . O. V., Gramatiuk, S. M., Tynynyka, L. M., Kozub, M. M., Taran, A. V., Nikolchenko, A. Y., & Prokopiuk, O. S. (2021). COMPARISON OF THERAPEUTIC EFFECTS OF DIFFERENT METHODS OF ADMINISTRATION OF MEZENCHIMAL STEM CELLS TO MICE WITH PREMATURE OVARIAN INSUFFICIENCY. Problems of Endocrine Pathology, 76(2), 35-40. https://doi.org/10.21856/j-PEP.2021.2.05