ovaries, sex hormones, conditioned environment, glial cell culture, neurotrophic factors, cryopreservation


Conditioned media from stem/progenitor cell cultures are a valuable source of growth factors for restoring ovarian function, inhibiting their premature aging, and preventing age-associated pathological changes in oocytes.

The aim of this work was to study the effect of conditioned media obtained from intact and cryopreserved cultures of glial cells on the structural and functional characteristics of the ovaries, the levels of the sex and gonadotropic hormones in rats of different ages. The experiments were carried out on females aged 6 (reproductive age, RA) and 14 months (late reproductive age, LRA). A monolayer culture in which glial cells predominated was obtained from the dorsal root ganglia of neonatal piglets. Cells were cryopreserved in the presence of 7.5% cryoprotectant dimethyl sulfoxide. Conditioned media from intact (ICM) and cryopreserved (CCM) cultures were fractionated by ultrafiltration and fractions up to 30 kDa were obtained. The ICM and CCM fractions were injected into rats intraperitoneally. The estrous cycle, the levels of gonadotropic and sex hormones, the histological structure of the ovaries and the quantitative composition of the follicles were studied.

ICM and CCM had a stimulating effect on the release of primordial follicles from the dormant state in the ovaries of RA and LRA animals, while the intraovarian follicular pool was rearranged with a decrease in the number of follicles of hormone-independent developmental stages (primordial and primary) and an increase in the number of atretic follicles. The introduction of ICM and CCM promoted an increase in the LH/FSH ratio and inhibition of the formation of age-associated cysts in LRA rats. The biological effect of CCM on the reproductive system of female rats was similar to that observed with the use of ICM.


Bellieni C. J Family Reprod Health 2016;10(3): 104-107.

Findlay JK, Hutt KJ, Hickey M, Anderson RA Biol Reprod 2015;93(5): 111. https://doi.org/10.1095/biolreprod.115.133652.

Streiter S, Fisch B, Sabbah B, Ao A, et al. Mol Human Reprod 2016;22(1): 3-17. https://doi.org/10.1093/molehr/gav057.

Kerr B, Garcia-Rudaz C, Dorfman M, et al. Reproduction 2009;138(1): 131-140. https://doi.org/10.1530/REP-08-0474.

Nilsson E, Dole G, Skinner MK. Reproduction 2009;138(4): 697-707. https://doi.org/10.1530/REP-09-0179.

Prokopyuk VYu, Karpenko VG, Shevchenko MV, et al. Innov Biosyst Bioeng 2020;4(3): 168-176. https://doi.org/10.20535/ibb.2020.4.3.215215.

Teixeira FG, Carvalho MM, Sousa N, Salgado AJ. Cell Mol Life Sci 2013;70(20): 3871-3882. https://doi.org/10.1007/s00018-013-1290-8.

Vizoso FJ, Eiro N, Cid S, et al. Int J Mol Sci 2017;18(9): 1852. https://doi.org/10.3390/ijms18091852.

Gwam C, Mohammed N, Ma X. Ann Transl Med 2021;9(1): 70. https://doi.org/10.21037/atm-20-5030.

Li HY, Say EH, Zhou XF. Stem Cells 2007;25(8): 2053-2065. https://doi.org/10.1634/stemcells.2007-0080.

Ali SG, Moisjejeva NM, Bozhok GA. Probl Cryobiol Cryomed 2020;30(2): 158-168. https://doi.org/10.15407/cryo30.02.158.

Walker MJ, Xu XM. Brain Sci 2018;8(6): 109. https://doi.org/10.3390/brainsci8060109.

Ruiz C, Casarejos MJ, Gomez A, et al. PLoS Curr 2012;2(4): e4fbca54a2028b. https://doi.org/10.1371/4fbca54a2028b.

Sengupta P. Int J Prev Med 2013;4(6): 624-630.

Dixon D, Alison R, Bach U, et al. J Toxicol Pathol 2014;27(3-4): 1S-107S.

Cruz G, Fernandois D, Paredes AH. Reproduction 2017;153(2): R59-R68. https://doi.org/10.1530/REP-16-0117.

Bahougne T, Angelopoulou E, Jeandidier N, Simonneaux V. Geroscience 2020;42(1): 323-331. https://doi.org/10.1007/s11357-019-00104-z.

Nesteruk HV, Kolot NV, Procenko OS, et al. Bull Probl Biol Med 2020;4(158): 173-178. https://doi.org/10.29254/2077-4214-2020-4-158-173-177.

Ford EA, Beckett EL, Roman SD, et al. Reproduction 2020;159(1): R15-R29. https://doi.org/10.1530/REP-19-0201.

Chen Y, Yang W, Shi X, et al. Front Cell Dev Biol 2020;8: 575706. https://doi.org/10.3389/fcell.2020.575706.

Steger RW, Peluso JJ. Exp Aging Res 1982;8(3-4): 203-208. https://doi.org/10.1080/03610738208260367

Ding C, Zou Q, Wang F, et al. Stem Cell Res Ther 2018;9(1): 55. https://doi.org/10.1186/s13287-018-0781-9.

Buigues A, Marchante M, de Miguel-Gómez L, et al. Am J Obstet Gynecol 2021;225(1): 65.E1-65.E14. https://doi.org/10.1016/j.ajog.2021.01.023.




How to Cite

Нестерук, Г., Алабедалькарім, Н., Колот, Н., Комаромі, Н., Проценко, О., & Легач, Є. . (2022). EFFECT OF CONDITIONED MEDIA FROM GLIAL CELL CULTURES ON THE REPRODUCTIVE SYSTEM OF FEMALE RATS OF DIFFERENT AGES. Problems of Endocrine Pathology, 79(2), 88-96. https://doi.org/10.21856/j-PEP.2022.2.13

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