THE IMPACT OF VITAMIN D AND/OR SAW PALMETTA BERRY EXTRACT ON THE SPERMOGRAMM AND SPERMATOZOA FUNCTIONING UNDER THE CONDITION OF EXPERIMENTAL BENIGN PROSTATIC HYPERPLASIA

Abstract

The tendency to the declining of number of spermatozoa in the ejaculate is detected in men worldwide. Benign prostatic hyperplasia (BPH) is one of the common pathology of men urogenital system. The infertility can be the consequence of this pathology. The significant number of side effects occurred during BHP medication encourages the development of new remedies for BHP prophylaxis and therapy. The laboratory animals are used in the experimental medicine with the aim to design pathological conditions. The Sulpiride model is often used for investigation of prostate gland protective activity of medicines in rats. This model’ essence lies in the prolonged sulpiride introduction which induces BPH development. The experimental and clinical investigations demonstrate questionable data as for vitamin D influence on the spermogram parameters.

Purpose. To determine the efficacy of cholecalciferol (vitamin D) administration, separately or together with Saw Palmetta berry extract, as for correction of spermogram in rats males under the condition of experimental sulpiride-induced benign prostatic hyperplasia.

Materials and methods. BPH has been modeled by intramuscular introduction of Sulpiride in dose of 40 mg/kg to 12-month rat males with body mass of 330-380 g during 30 days. The correction of BPH was carried out by vitamin D₃ in dose of 4000 IU or saw Palmetta berry extract in dose of 35 mg/kg, separately or together, during 21 days after last injection of Sulpiride. Control group of animals have simultaneously received injection of Sulpiride and 0,9% solution of Sodium Chloride in dose of 0.5 mL. The spermatogenesis (sperms concentration, the percentage of motile and dead cells) and functional capacity of gametes have been evaluated in the suspension of spermatozoa obtained from epididymides of decapitated males. The probability of mean deviation has been determined according to t-Student test. Data were performed as arithmetic mean () and standard error of the mean (±S). The differences were considered to be significant at Р<0.05.

Results. It has been determined that Sulpiride-induced BPH has caused statistically significant changes of all spermogram’ parameters which appears in the declined concentration and motility of gametes, in the increased number of pathological and dead forms of sperms comparing to Control group.

The correction of spermatogenesis disturbances by cholecalciferol or reference medicine has led to statistically significant increase in motile sperms percentage almost by two times and to the decrease in number of dead cells almost by two times in rats with BPH comparing to nontreated BPH group. Simultaneous use of vitamin D₃ with reference medicine has caused the increasing by 1.7 times of sperms motility, gametes concentration by 1.5 times, the concentration of morphologically normal cells by 1.6 times and decreasing of number of dead cells almost two times in rats with experimental pathology comparing to BPH group.

Conclusions. Sulpiride-induced benign prostatic hyperplasia causes the disturbance of spermatogenesis and functional capacity of spermatozoa in 12-month rat males. The correction of this pathology by cholecalciferol has improved sperm cells motility and has declined the percentage of dead cells. The same effect has been observed after administration of Saw Palmetta Berry extract. Simultaneous administration of these medicines has increased spermatozoa concentration, the number of morphologically normal gametes, sperms motility and has declined the percentage of dead sperm cells in rats with experimental benign prostatic hyperplasia.