VITAMIN D AND SAW PALMETTO FRUIT EXTRACT IN EXPERIMENTAL PROSTATE PATHOLOGY

Inna Bielkina; Natalia Smolienko; Yevheniia Korenieva; Ihor Marakhovskyi; Natalia Tkachenko; Olena Protsenko; Roman Blahoveshchenskyi; Volodymyr Bondarenko; Katerina Misiura

doi:10.21856/j-PEP.2025.3.03

VITAMIN D AND SAW PALMETTO FRUIT EXTRACT IN EXPERIMENTAL PROSTATE PATHOLOGY

Authors

Bielkina I. O. SI «V. Danilevsky Institute for Endocrine Pathology Problems of the NAMS of Ukraine», Kharkiv, Ukraine https://orcid.org/0000-0003-0439-0969
Smolienko N. P. SI «V. Danilevsky Institute for Endocrine Pathology Problems of the NAMS of Ukraine», Kharkiv, Ukraine https://orcid.org/0000-0003-2064-8608
Korenieva Ye. M. SI «V. Danilevsky Institute for Endocrine Pathology Problems of the NAMS of Ukraine», Kharkiv, Ukraine https://orcid.org/0000-0002-4570-6563
Marakhovskyi I. O. SI «V. Danilevsky Institute for Endocrine Pathology Problems of the NAMS of Ukraine», Kharkiv, Ukraine https://orcid.org/0000-0002-9744-8324
Tkachenko N. О. V. N. Karazin Kharkiv National University, Kharkiv, Ukraine https://orcid.org/0000-0001-5500-3303
Protsenko O. S. V. N. Karazin Kharkiv National University https://orcid.org/0000-0001-6998-9783
Blahoveshchenskyi R. Ye. Kharkiv National Medical University, Kharkiv, Ukraine https://orcid.org/0000-0003-3022-8180
Bondarenko V. O. SI «V. Danilevsky Institute for Endocrine Pathology Problems of the NAMS of Ukraine», Kharkiv, Ukraine https://orcid.org/0000-0002-9254-3875
Misiura K. V. SI «V. Danilevsky Institute for Endocrine Pathology Problems of the NAMS of Ukraine», Kharkiv, Ukraine https://orcid.org/0000-0002-0258-9109

DOI:

https://doi.org/10.21856/j-PEP.2025.3.03

Keywords:

benign prostatic hyperplasia, vitamin D, saw palmetto fruit extract, male rats, testosterone, sexual behavior

Abstract

Benign prostatic hyperplasia is a serious problem that significantly reduces the quality of life of men, so the search for new prostatoprotectors is highly relevant.
The aim: to evaluate the effect of vitamin D and saw palmetto fruit extract on prostate mass, sex hormone levels, and sexual behavior under experimental conditions of prostate pathology.
Materials and methods. Prostate pathology was induced by administration of sulpiride solution at a dose of 40 mg/kg b.w. to 12-month-old male rats for 30 days (BPH group). For correction, the following treatments were administered for 21 days per os: vitamin D3 at a dose of 4000 IU (BPH+vit.D group); Sabal palm fruit extract at a dose of 35 mg/kg b.w. (BPH+Ref group); or vitamin D3 and Sabal palm fruit extract at the same doses (BPH+vit.D+Ref group) were administered together. Sexual behavior, genital organ mass, and serum testosterone (T) and estradiol (E2) levels were determined. The probability of differences in mean values was determined by Student's t test. The χ2 test was used for comparative analysis of relative values. Differences were considered significant at p < 0.05.
Results. In the BPH group, the prostate mass increased, and in the BPH+Vit.D+Ref group, the organ mass normalized. Serum total T levels in the BPH group decreased by 1.66 times, and the T/E2 ratio was 1.53 times lower than in the control group. When using corrective substances, the T concentration normalized. The T/E2 ratio increased by 1.5 times. In the BPH group, both the courtship and copulatory components of sexual behavior were impaired, and no male in this group ejaculated. In the BPH+Ref group, the latency period for the onset of mounts (by 2.7 times) and intromissions (by 1.7 times) decreased, the number of courtship behaviors decreased by 1.9 times, compared to the BPH group. In animals in the BPH+vit.D+Ref group, the latency period for mounts decreased by 3.3 times, intromissions - by 4.4 times, the number of mounts and intromissions increased by 2.2 and 2.9 times compared to the BPH group. Ejaculations were recorded in 40% of males in this group.
Conclusions. Experimental sulpiride-induced prostatic hyperplasia is accompanied by an increase in the mass of prostate, an imbalance of hormonal ratios, and suppression of sexual behavior parameters. The combined use of vitamin D and saw palmetto fruit extract corrects BPH, promotes normalization of prostate mass, an increase in testosterone levels, and restores the ratio of sex hormones, and improves both the courtship and copulatory components of the sexual behavior in rats.

References

1. Zheng C, Luo Y, Chen Y, et al. Experim Ther Med 2020;19(4): 2551-2562. https://doi.org/10.3892/etm.2020.8521.

2. Brechka NM, Bondarenko VO, Shcherbak OV, Korenieva YM. Wiadomosci Lekarskie 2024;77(1): 135-143. https://doi.org/10.36740/wlek202401117.

3. Awedew AF, Han H, Abbasi B, et al. The Lancet Healthy Longevity 2022;3(11): e754-e776. https://doi.org/10.1016/S2666-7568(22)00213-6.

4. Pender GC, Ombaka JH, Mwitari PG, Guyah B. Int J Sci Res Arch 2024;11(2): 049-074. https://doi.org/10.30574/ijsra.2024.11.2.0381.

5. Nastanova 00246. Dobrojakisna giperplazija peredmihurovoi' zalozy (DGPZ), 2017: 10 р, available at: https://guidelines.moz.gov.ua/documents/3132

6. Blair HA. Drugs & Aging 2022;39(3): 235-243. https://doi.org/10.1007/s40266-022-00924-3.

7. Bruskewitz RC. Rev Urol 2003;5(2): 72-80, available at: https://reviewsinurology.com/issues/vol/5/no/2/quality-of-life-and-sexual-function-in-patients-with-benign-prostatic-hyperplasia

8. Zendehdel A, Ansari M, Khatami F, et al. Clin Nutr 2021;40(5): 3325-3331. https://doi.org/10.1016/j.clnu.2020.11.005.

9. Angrimani DS, Brito MM, Rui BR, et al. Sci Reports 2020;10(1): 14834. https://doi.org/10.1038/s41598-020-71691-7.

10. Chen Y, Xu H, Liu C, et al. Disease Markers 2021;2021(1): 4029470. https://doi.org/10.1155/2021/4029470.

11. Couteau N, Duquesne I, Frédéric P, et al. J Clin Med 2021;10(24): 5788. https://doi.org/10.3390/jcm10245788.

12. Zhuang B, Zhuang C, Jiang Y, et al. Andrology 2024;2024: 1-13. https://doi.org/10.1111/andr.13778.

13. Corona G, Maggi M. Rev Endocrine Metab Dis 2022;23(6): 1159-1172. https://doi.org/10.1007/s11154-022-09748-3.

14. Van Coppenolle F. The Prostate 2000;43(1): 49-58. https://doi.org/10.1002/(SICI)1097-0045(20000401)43:1<49::AID-PROS7>3.0.CO;2-J

15. Geavlete P, Multescu R, Geavlete B. Ther Adv Urol 2011;3(4): 193-198. https://doi.org/10.1177/1756287211418725.

16. Chung A, Kini M, Hartigan SM, et al. Academic Press 2021: 107-111. https://doi.org/10.1016/B978-0-12-819765-3.00008-X.

17. Espinosa G, Esposito R, Kazzazi A, Djavan B. Can J Urol 2013;20(4): 6820-6825, available at: https://canjurol.com/html/free-articles/V20I4_03F_DrEspinosa.pdf

18. Vázquez-Lorente H, Herrera-Quintana L, Jiménez-Sánchez L, et al. Antioxidants 2024;13(8): 996. https://doi.org/10.3390/antiox13080996.

19. Abu-Zaid A, Saleh SA, Adly HM, et al. Diseases 2024;12(10): 228. https://doi.org/10.3390/diseases12100228.

20. Canguven O, Talib RA, El Ansari W, et al. The Aging Male 2017;20(1): 9-16. https://doi.org/10.1080/13685538.2016.1271783.

21. Huang Y, Jin H, Chen J, et al. Animal Reprod Sci 2015;158: 109-114. https://doi.org/10.1016/j.anireprosci.2015.05.008.

22. Crafa A, Cannarella R, Barbagallo F, et al. Biomolecules 2023;13(6): 930. https://doi.org/10.3390/biom13060930.

23. Corona G, Isidori AM, Aversa A, et al. J Sex Med 2016;13(3): 317-337. https://doi.org/10.1016/j.jsxm.2016.01.007.

24. Chen Z, Jiang J, Jiang R. J Sex Med 2024;21(8): 663-670. https://doi.org/10.1093/jsxmed/qdae071.

25. Yafi FA, Jenkins L, Albersen M, et al. Nature Rev Dis Primers 2016; 2(1): 1-20. https://doi.org/10.1038/nrdp.2016.3.

26. Tirabassi G, Sudano M, Salvio G, et al. Int J Endocrinol 2018;2018(1): 3720813. https://doi.org/10.1155/2018/3720813.

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Published

2025-09-15

How to Cite

Bielkina, I., Smolienko, N., Korenieva, Y., Marakhovskyi, I., Tkachenko, N., Protsenko, O., … Misiura, K. (2025). VITAMIN D AND SAW PALMETTO FRUIT EXTRACT IN EXPERIMENTAL PROSTATE PATHOLOGY. Problems of Endocrine Pathology, 82(3), 20–28. https://doi.org/10.21856/j-PEP.2025.3.03