PECULIARITIES OF ANTIOXIDANT STATUS IN BRAIN STRUCTURES IN RATS OF DIFFERENT AGES UNDER CONDITIONS OF EXPERIMENTAL HYPERTHYROXINEMIA
DOI:
https://doi.org/10.21856/j-PEP.2023.2.07Keywords:
antioxidant system, rats, thyroid hormones, experimental hyperthyroxinemia, brain, thyroid gland, age-related changesAbstract
The purpose of this work was to analyze the neurochemical features of the component composition of the antioxidant system in brain structures under conditions of experimental hyperthyroxinemia, which are formed under the adverse influence of anxiety factors.
Materials and methods. The study was conducted on Wistar rats (n=50). The animals were represented by two age groups: 100-110 days (young rats), weighing 90-270 g, 7-8 months (middle-aged rats), weighing 210-340 g. Each age group of animals was divided into intact (control) and experimental (hyperthyroxinemia). Experimental hyperthyroxinemia was modeled by adding homogenized tablets of L-thyroxine (20 μg/day, Berlin-Chemie AG, Germany) at a fixed time (9:00 a.m.) with the same portion of food. On the 14th day, hyperthyroxinemia was registered in the animals with a thyroxine level in peripheral blood higher than 10 μg/dL (the results were verified by enzyme immunoassay using the Humarlander test system). After conducting behavioral tests, rats were selected for determination and evaluation of biochemical indicators (catalase and glutathione peroxidase) in the cortex, stem, and hippocampus of the brain using spectrophotometric methods.
Results and conclusions. It was established that experimental hyperthyroxinemia against the background of anxiety causes significant changes in the activity of antioxidant enzymes in the cortex, stem, and hippocampus of the brain of white rats of various ages. Age-related changes in the activity of antioxidant components that occur during the maturation of the nervous system are recorded. The analysis of biochemical features of enzymatic activity in the brain confirms the age-related antioxidant effect involving glutathione peroxidase in middle-aged rats. The need to conduct further studies aimed at determining the pathological impact of oxidative stress under conditions of hyperthyroidism and anxiety on the metabolic status of the central nervous system at different age stages is substantiated.
References
Guerri G, Bressan S, Sartori M, et al. Acta Biomed 2019;90(10-S): 83-86. https://doi.org/10.23750/abm.v90i10-S.8765.
Taylor PN, Albrecht D, Scholz A, et al. Nat Rev Endocrinol 2018;14(5): 301-316. https://doi.org/10.1038/nrendo.2018.18.
Khoo B, Tan T, Clarke SA, Mills EG, et al. J Clin Endocrinol Metab 2021;106(2): e803-e811. https://doi.org/10.1210/clinem/dgaa830.
The Thyroid and Its Diseases. Basis of Thyroid Disease: Epidemiology of Thyroid Disorders. https://doi.org/10.1007/978-3-319-72102-6_1.
Lucia FS, Pacheco-Torres J, Gonzalez-Granero S, et al. Front Neuroanat 2018;9: 9. https://doi.org/10.3389/fnana.2018.00031.
Pankiv VI. Int J Endocrinol 2021;13(2): 147-151. https://doi.org/10.22141/2224-0721.13.2.2017.100604.
Khaleghzadeh-Ahangar H, Talebi A, Mohseni-Moghaddam P. Neuroendocrinology 2022;112(9): 835-844. https://doi.org/10.1159/000521650.
Cheng SY, Leonard JL, Davis PJ. Endocr Rev 2010;31(2): 139-170. https://doi.org/10.1210/er.2009-0007.
Bernal J. J Endocrinol 2017;232(2): 83-97. https://doi.org/10.1530/JOE-16-0424.
Navarro D, Alvarado M, Navarrete F, et al. Front Neuroanat 2015;9: 9. https://doi.org/10.3389/fnana.2015.00009.
Chuhay OO, Lubinets LA Medychna ta Klinichna Khimiia 2016;4: 73-76. https://doi.org/10.11603/mcch.2410-681X.2016.v0.i4.7264.
Tiazhka OV, Zahorodnia YaM. Perynatolohiya i Pediatriia 2016;2(66): 101-105. https://doi.org/10.15574/PP.2016.66.101.
Regeda-Furdychko MM. ZKEM 2019;(3): 124-128. https://doi.org/10.11603/1811-2471.2019.v.i3.10524.
Rol tyreoidnykh hormoniv v realizatsii fiziolohichnykh mekhanizmiv vrodzhenykh ta nabutykh form povedinky u shchuriv riznoho viku, available at: https://repo.dma.dp.ua/221/.
Kopanytsia OM. ZKEM 2018;4. https://doi.org/10.11603/1811-2471.2017.v0.i4.8378.
Nerush PO, Demchenko OM. Tavrychnyi Med-Biol Visn 2004;7(7): 55-59.
Lishnevskaya V, Bruzgina T, Korkushko O. Fiziol Zh 2003;49(6): 47-51.
Marchioli R, Barzi F, Bomba E, et al. Circulation 2002;105(16): 1897-1903. https://doi.org/10.1161/01.CIR.0000014682.14181.F2.
Lands WEM. Eur Heart J 2001;3(Suppl D): 22-25. https://doi.org/10.1016/S1520-765X(01)90114-2.
Nerush PO, Demchenko OM. Fiziol Zh 2005;51(3): 67-72.
Demchenko OM, Rodinsky OG, Kozlova YuV, et al. Prob Endocrine Pathol 2022;79(1): 85-92. https://doi.org/10.21856/j-PEP.2022.1.12.
Zinych O. Mizhnar Endokrynol Zhurn 2021;17(1): 47-49.
Basysta KI, Rodinskiy AG, Demchenko OМ, Guz LV. Bull Probl Biol Med 2021;161(3): 356-359. https://doi.org/10.29254/2077-4214-2021-3-161-356-359
The Development of Science-based Guidelines for Laboratory Animal Care: Proceedings of the November 2003 International Workshop. International Guiding Principles for Biomedical Research Involving Animals, available at: https://www.ncbi.nlm.nih.gov/books/NBK25438/.
Chekman IS, Bjelenichev IF, Nagorna OO. Doklinichne vyvchennja specyfichnoi aktyvnosti potencijnyh likarskyh zasobiv pervynnoi ta vtorynnoi nejroprotekcii, Kyiv, 2016: 93 p.
Verkhohliad IM, Tsurzevych BO, Kudriashov YuB. Radiologia 1991;31(5): 668-672.
Koroliuk M. Laboratorna Sprava 1988;(1): 16-29.
Hadwan MH, Ali SK. Anal Biochem 2018;542: 29-33. https://doi.org/10.1016/j.ab.2017.11.013
Djordjević VV, Kostić J, Krivokapić Ž, et al. Medicina 2022;58(10): 1491. https://doi.org/10.3390/medicina58101491.
Bagmut I, Pogorelov V, Zhukov V. Int Neurol J 2020;16(3): 30-35. https://doi.org/10.22141/2224-0713.16.3.2020.203447.
Razyhraiev AV, Yushyna AD, Tytovych IA. Bull Exp Biol Med 2018;165(2): 292-295. https://doi.org/10.1007/s10517-018-4151-5.
Lang TA. Yak opysuvaty statystyku v medytsyni. Kerivnytstvo dlia avtoriv, redaktoriv i retsenzentiv, Moskva, 2011: 480 p.
Voronenko YuV. Sotsialna medytsyna ta orhanizatsiia okhorony zdorovia, Ternopil, 2002: 332 p.
Lang TA, Altman DG. Int J Nurs Stud 2015;52(1): 5-9. https://doi.org/10.1016/j.ijnurstu.2014.09.006.
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