THE STATE OF SPERMATOGENESIS OF RATS IN TERMS OF EXPERIMENTAL HEPATITIS OF DIFFERENT GENESIS AND ITS CORRECTION WITH A NEW REMEDY
DOI:
https://doi.org/10.21856/j-PEP.2025.2.05Keywords:
experimental hepatitis, paracetamol, spermatogenesis disorders, dicarboxylic acid derivative, selenium, new agentAbstract
Background. One of the important and urgent tasks of modern endocrinology is the study of disorders of the male reproductive system in the presence of somatic pathologies, in particular liver damage of various genesis. The aim of the study was to determine biomarkers of spermatogenesis disorders in the setting of toxic and drug-induced hepatitis and their correction with a new drug.
Materials and methods. The study was conducted on white, sexually mature male rats weighing 200-250 g. The animals were divided into groups: control, control-hepatitis, rats with hepatitis (toxic or drug-induced), which received correction with a new agent based on a dicarboxylic acid derivative and a selenium-containing compound orally daily at a dose of 32 mg/kg body weight for a month. Some parameters of the spermogram, the level of free testosterone in blood serum, the fructose content of the epididymis homogenate, and the histological condition of the testicles were studied. The nature of the distribution of the trait in the sample was determined using the Shapiro-Wilk test (W).
Results. In conditions of toxic and drug-induced hepatitis, a decrease in the level of free testosterone in the blood serum, fructose content in the epididymis homogenate in animals of the “hepatitis” group compared to control animals, and negative changes in spermogram indicators were noted: a decrease in the total concentration of spermatozoa, the number of morphologically normal forms, an increase in the percentage of pathological cells and a decrease in their mobility. The use of a new agent for the correction of liver disorders led to the restoration of spermogram indicators, an increase in the level of testosterone and fructose content
Conclusions. Under the conditions of toxic and drug-induced hepatitis disorders of the functional state of the liver develop in the body of rats, resulting in inhibition of spermatogenesis processes, the main biomarkers of which are the decrease in the level of free testosterone in the blood serum and the fructose content in the epididymis homogenate. The use of the new drug contributes to the normalization of functional and metabolic processes in the liver, as a result of which spermatogenesis indicators and the histostructure of the testicles are restored.
References
1. Horpynchenko I I, Romaniuk M H. Zdorove Muzhchynу 2016;1: 8-17. https://nbuv.gov.ua/UJRN/Zdmu_2016_1_3
2. Kudrya MYa, Melnykivska NV, Ustenko NV, et al. Probl Endokryn Patol 2015;4: 53-60. https://doi.org/10.21856/j-PEP.2015.4.07
3. Ryan C, Petering Nathan, Brooks А. Am Fam Physician 2017;96(7): 441-449.
4. Melnykivska NV, Kudria MYa, Palahina IA, et al. Probl Endokryn Patol 2020;4: 131-137. https://doi.org/10.21856/j-PEP.2020.4.17
5. Nelson D. Osnovy byokhymyy Lenyndzhera. Vol. 2. Byoenerhetyka ta meta-bolyzm, Moskva, 2014: 636 p.
6. Metabolic pathways of fructose and galactose conversion; biological signifi-cance, hereditary enzymopathies of their metabolism, available at : https://doctrina.space/subjects/biochemistry/info_25
7. Patent № 38/30 A UA. 3 (4,5-dyhidrotiazol-2-il) amid tsys-1,2,2-trymetyltsyklopentan-1,3-dykarbonovoi kysloty, shcho stymuliuie spermato-henez.
8. Stefanov AV. Doklynycheskye yssledovanyia lekarstvennykh sredstv, Kyiv, 2002: 568 p.
9. Kiernan J. A. Histological and histochemical methods: theory and practice, Banbury, 2015: 571 p.
10. Atramentova LA, Utevskaia OM. Statystycheskye metody v byolohyy, Hor-lovka, 2008: 248 p.
11. Moroziuk AY, Melnykivska NV, Kudrya MYa, et al. Farmakolohiia ta Likarska Toksykolohiia 2023;17(5): 318-327. https://doi.org/10.33250/17.04.318
12. Yunusova S, Rozhkovskyi Ya, Prystupa B, Bohatu S. Fitoter Chasopys – Phy-tother J 2022;3: 78-85. https://doi.org/10.33617/2522-9680-2022-3-78
13. Bolshukhyn SYu, Peretiahyn SP, Martusevych AK, Soloveva AH. Byoradykaly ta antyoksydanty 2014;1: 59-65.
14. Morozyuk A, Kudrya M, Melnikovskaya N, et al. ScienceRise: Biological Science 2024;3(40): 32-38. https://doi.org/10.15587/2519-8025.2024.320788
15. Fakhryddynovych YoY, Razhabovych B. Sci J Appl Med Sciences 2023;2(5): 381-383. https://sciencebox.uz/index.php/amaltibbiyot/article/view/7385
16. Allaire M, Rautou PE, Codogno P, Lotersztajn S. J Hepatol 2019;70: 985-998. https://doi.org/10.1016/j.jhep.2019.01.026
17. Raja Gopal Reddy Mooli, Dhanunjay Mukhi, Sadeesh K. Ramakrishnan. Compr Physiol 2022;12(2): 3167-3192. https://doi.org/10.1002/cphy.c200021
18. Rungratanawanich W, et al. Redox Biol 2023;59: 102577. https://doi.org/10.1016/j.redox.2022.102577
19. Yang YM, Cho YE, Hwang S. Int J Mol Sci 2022;23(2): 774. https://doi.org/10.3390/ijms23020774
20. Rotundo L, Pyrsopoulos N. World J Hepatol 2020;12(4): 125-136. https://doi.org/10.4254/wjh.v12.i4.125
21. Goetzman ES, et al. J Clin Invest 2024;134(12): e174186. https://doi.org/10.1172/JCI174186
22. Silva Barbosa AC, et al. J Am Soc Nephrol 2024;35(2): 135-148. https://doi.org/10.1681/ASN.0000000000000266
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2025 Problems of Endocrine Pathology
This work is licensed under a Creative Commons Attribution 4.0 International License.
ISSN 
ISSN 
